Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Xiao-Qing-Long-Tang Maintains Cardiac Function during Heart Failure with Reduced Ejection Fraction in Salt-Sensitive Rats by Regulating the Imbalance of Cardiac Sympathetic Innervation

doi: 10.1155/2020/9467271

Figure Lengend Snippet: Analysis of the expression of NA, NGF, and LIF. (a), (b) Detection of NA concentrations in left ventricle and plasma by ELISA, respectively. (c) Representative Western blot of myocardial NGF. (d) Relative protein level was calculated by band intensity against β -actin. (e) The mRNA expressions of NGF in left ventricle tissues were determined by qRT-PCR. The levels of myocardial LIF were measured by (f) Western blot, (g) band intensity against β -actin, and (h) qRT-PCR. Quantitative data are shown as the mean ± SD, n = 9, ∗ P < 0.05, ∗∗ P < 0.01 vs the SS-13 BN group; # P < 0.05, ## P < 0.01 vs. the DS group.

Article Snippet: The membranes were blocked with 5% nonfat milk in TBST for 1 h at room temperature and, subsequently, incubated overnight at 4°C with primary antibodies against NGF (Abcam, Cat No. ab52918, 1 : 1000), LIF (Abcam, Cat No. ab113262, 1 : 1000), TH (Abcam, Cat No. ab137869, 1 : 5000), CHAT (Abcam, Cat No. ab178850, 1 : 1000), growth-associated protein 43 (GAP43) (Abcam, Cat No. ab16053, 1 : 1000), and β -actin (Proteintech, Cat No. 20536-1-AP, 1 : 5000).

Techniques: Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Xiao-Qing-Long-Tang Maintains Cardiac Function during Heart Failure with Reduced Ejection Fraction in Salt-Sensitive Rats by Regulating the Imbalance of Cardiac Sympathetic Innervation

doi: 10.1155/2020/9467271

Figure Lengend Snippet: Treatment with XQLT regulated the anatomical dominance of sympathetic nerves. (a) Double immunofluorescence of TH+/GAP43+ positive fibers in left ventricle (scale bars:50 μ m). (b) Relative TH+/GAP43+ positive fibers area. (c) Representative Western blot of myocardial GAP43. (d) Relative protein level was calculated by band intensity against β -actin. Quantitative data are shown as the mean ± SD, n = 9. ∗∗ P < 0.01 vs. the SS-13 BN group; ## P < 0.01 vs. the DS group.

Article Snippet: The membranes were blocked with 5% nonfat milk in TBST for 1 h at room temperature and, subsequently, incubated overnight at 4°C with primary antibodies against NGF (Abcam, Cat No. ab52918, 1 : 1000), LIF (Abcam, Cat No. ab113262, 1 : 1000), TH (Abcam, Cat No. ab137869, 1 : 5000), CHAT (Abcam, Cat No. ab178850, 1 : 1000), growth-associated protein 43 (GAP43) (Abcam, Cat No. ab16053, 1 : 1000), and β -actin (Proteintech, Cat No. 20536-1-AP, 1 : 5000).

Techniques: Immunofluorescence, Western Blot

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Xiao-Qing-Long-Tang Maintains Cardiac Function during Heart Failure with Reduced Ejection Fraction in Salt-Sensitive Rats by Regulating the Imbalance of Cardiac Sympathetic Innervation

doi: 10.1155/2020/9467271

Figure Lengend Snippet: XQLT improved the functional dominance of sympathetic nerves. (a) Double immunofluorescence of TH+/CHAT + positive fibers in the left ventricle (scale bars: 50 μ m). (b) Relative TH+/CHAT + positive fibers area. (c) Representative Western blot of myocardial TH and CHAT. (d) and (e) Relative protein levels of TH and CHAT were calculated by band intensity against β -actin. (f) The SGVs and SAGVs of left ventricles by transmission electron microscopy (black arrows indicate the SGV and white arrows indicate the SAGV, scale bars: 500 nm). Quantitative data are shown as the mean ± SD, n = 9. ∗∗ P < 0.01, ## P < 0.01 vs. the SS-13 BN group; # P < 0.05 vs. the DS group.

Article Snippet: The membranes were blocked with 5% nonfat milk in TBST for 1 h at room temperature and, subsequently, incubated overnight at 4°C with primary antibodies against NGF (Abcam, Cat No. ab52918, 1 : 1000), LIF (Abcam, Cat No. ab113262, 1 : 1000), TH (Abcam, Cat No. ab137869, 1 : 5000), CHAT (Abcam, Cat No. ab178850, 1 : 1000), growth-associated protein 43 (GAP43) (Abcam, Cat No. ab16053, 1 : 1000), and β -actin (Proteintech, Cat No. 20536-1-AP, 1 : 5000).

Techniques: Functional Assay, Immunofluorescence, Western Blot, Transmission Assay, Electron Microscopy